fa 11 bio rad Search Results


cd68  (Novus Biologicals)
94
Novus Biologicals cd68
Systemic AAV dissemination, eGFP expression in Schwann cells and inflammation at the site of AAV delivery using SciNDi (A) Dual-IF detection of eGFP and neurofilament light chain (NF-L) in the sciatic nerve trunk following bilateral IM application of PBS (vehicle). Representative images showing eGFP alone (green), NF-L (red), the merge images of eGFP and NF-L, and nuclear marker DAPI (blue). IF procedures and image acquisition were performed as described under E and 2F (nerve trunk and 10× magnified views). (B) Immunohistochemical (IHC) detection of eGFP in the right lumbar L4/L5 spinal ventral horn (VH-R) or both dorsal horns (DH-R and DH-L) after AAV delivery using SciNDi. IHC procedures and image acquisition were performed as described under G–2I. (C) qRT-PCR determination of vector genome copy numbers (AAV2-ITR/2N∗β- tubulin) comparing AAV delivery via IM route or SciNDi (Mean ± s.e.m.; SC: lumbar spinal cord, n = 6; B: whole brain, n = 4–6; and L: liver, n = 6; One- way ANOVA; ns, not significant). (D) qRT-PCR determination of eGFP (transgene) expression (ΔΔCT, relative to PBS injected cohorts). Statistics in A-B (Mean ± s.e.m.; SC: lumbar spinal cord, n = 6; B: whole brain, n = 4–6; and L: liver, n = 6; Mann-Whitney test: ∗= p ≤ 0.05, ns = not significant). (E and F ) Dual-IF co-detection of eGFP in sciatic nerve along with either the Schwann cell marker (S100, in E), or monocyte/macrophage marker <t>(CD68,</t> in F) following bilateral IM application of PBS, and AAV delivery using IM injection or SciNDi. Representative images (20× views) showing eGFP alone (green), S100 alone (red) (in E) or <t>CD68</t> alone (red) (in F), and the respective merge images along with the nuclear marker DAPI (blue). Antibodies: eGFP (abcam #13970ab, Polyclonal chicken, 1:200), S100 (DAKO #Z0311, rabbit polyclonal, 1:200), CD68 (Novus Biologicals <t>#NBP2-33337,</t> rat mAb FA-11, 1:200). High resolution IF images were acquired using a Zeiss observer inverted microscope equipped with colibri 7 LED illumination. (G–I) Dual-IF co-detection of eGFP and and monocyte/macrophage marker (CD68) in hindlimb femoris muscle following PBS application using SciNDi (in G), and AAV delivery using IM injection (in H) or SciNDi (in I). Representative images (20× view, merge with DAPI, IF procedures and image acquisition were as described under <xref ref-type=Figure 3 F). " width="250" height="auto" />
Cd68, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd68/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
cd68 - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
ion retardation resin  (Bio-Rad)
93
Bio-Rad ion retardation resin
Systemic AAV dissemination, eGFP expression in Schwann cells and inflammation at the site of AAV delivery using SciNDi (A) Dual-IF detection of eGFP and neurofilament light chain (NF-L) in the sciatic nerve trunk following bilateral IM application of PBS (vehicle). Representative images showing eGFP alone (green), NF-L (red), the merge images of eGFP and NF-L, and nuclear marker DAPI (blue). IF procedures and image acquisition were performed as described under E and 2F (nerve trunk and 10× magnified views). (B) Immunohistochemical (IHC) detection of eGFP in the right lumbar L4/L5 spinal ventral horn (VH-R) or both dorsal horns (DH-R and DH-L) after AAV delivery using SciNDi. IHC procedures and image acquisition were performed as described under G–2I. (C) qRT-PCR determination of vector genome copy numbers (AAV2-ITR/2N∗β- tubulin) comparing AAV delivery via IM route or SciNDi (Mean ± s.e.m.; SC: lumbar spinal cord, n = 6; B: whole brain, n = 4–6; and L: liver, n = 6; One- way ANOVA; ns, not significant). (D) qRT-PCR determination of eGFP (transgene) expression (ΔΔCT, relative to PBS injected cohorts). Statistics in A-B (Mean ± s.e.m.; SC: lumbar spinal cord, n = 6; B: whole brain, n = 4–6; and L: liver, n = 6; Mann-Whitney test: ∗= p ≤ 0.05, ns = not significant). (E and F ) Dual-IF co-detection of eGFP in sciatic nerve along with either the Schwann cell marker (S100, in E), or monocyte/macrophage marker <t>(CD68,</t> in F) following bilateral IM application of PBS, and AAV delivery using IM injection or SciNDi. Representative images (20× views) showing eGFP alone (green), S100 alone (red) (in E) or <t>CD68</t> alone (red) (in F), and the respective merge images along with the nuclear marker DAPI (blue). Antibodies: eGFP (abcam #13970ab, Polyclonal chicken, 1:200), S100 (DAKO #Z0311, rabbit polyclonal, 1:200), CD68 (Novus Biologicals <t>#NBP2-33337,</t> rat mAb FA-11, 1:200). High resolution IF images were acquired using a Zeiss observer inverted microscope equipped with colibri 7 LED illumination. (G–I) Dual-IF co-detection of eGFP and and monocyte/macrophage marker (CD68) in hindlimb femoris muscle following PBS application using SciNDi (in G), and AAV delivery using IM injection (in H) or SciNDi (in I). Representative images (20× view, merge with DAPI, IF procedures and image acquisition were as described under <xref ref-type=Figure 3 F). " width="250" height="auto" />
Ion Retardation Resin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ion retardation resin/product/Bio-Rad
Average 93 stars, based on 1 article reviews
ion retardation resin - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier

Image Search Results


Systemic AAV dissemination, eGFP expression in Schwann cells and inflammation at the site of AAV delivery using SciNDi (A) Dual-IF detection of eGFP and neurofilament light chain (NF-L) in the sciatic nerve trunk following bilateral IM application of PBS (vehicle). Representative images showing eGFP alone (green), NF-L (red), the merge images of eGFP and NF-L, and nuclear marker DAPI (blue). IF procedures and image acquisition were performed as described under E and 2F (nerve trunk and 10× magnified views). (B) Immunohistochemical (IHC) detection of eGFP in the right lumbar L4/L5 spinal ventral horn (VH-R) or both dorsal horns (DH-R and DH-L) after AAV delivery using SciNDi. IHC procedures and image acquisition were performed as described under G–2I. (C) qRT-PCR determination of vector genome copy numbers (AAV2-ITR/2N∗β- tubulin) comparing AAV delivery via IM route or SciNDi (Mean ± s.e.m.; SC: lumbar spinal cord, n = 6; B: whole brain, n = 4–6; and L: liver, n = 6; One- way ANOVA; ns, not significant). (D) qRT-PCR determination of eGFP (transgene) expression (ΔΔCT, relative to PBS injected cohorts). Statistics in A-B (Mean ± s.e.m.; SC: lumbar spinal cord, n = 6; B: whole brain, n = 4–6; and L: liver, n = 6; Mann-Whitney test: ∗= p ≤ 0.05, ns = not significant). (E and F ) Dual-IF co-detection of eGFP in sciatic nerve along with either the Schwann cell marker (S100, in E), or monocyte/macrophage marker (CD68, in F) following bilateral IM application of PBS, and AAV delivery using IM injection or SciNDi. Representative images (20× views) showing eGFP alone (green), S100 alone (red) (in E) or CD68 alone (red) (in F), and the respective merge images along with the nuclear marker DAPI (blue). Antibodies: eGFP (abcam #13970ab, Polyclonal chicken, 1:200), S100 (DAKO #Z0311, rabbit polyclonal, 1:200), CD68 (Novus Biologicals #NBP2-33337, rat mAb FA-11, 1:200). High resolution IF images were acquired using a Zeiss observer inverted microscope equipped with colibri 7 LED illumination. (G–I) Dual-IF co-detection of eGFP and and monocyte/macrophage marker (CD68) in hindlimb femoris muscle following PBS application using SciNDi (in G), and AAV delivery using IM injection (in H) or SciNDi (in I). Representative images (20× view, merge with DAPI, IF procedures and image acquisition were as described under <xref ref-type=Figure 3 F). " width="100%" height="100%">

Journal: STAR Protocols

Article Title: Recombinant adeno-associated virus mediated gene delivery in the extracranial nervous system of adult mice by direct nerve immersion

doi: 10.1016/j.xpro.2022.101181

Figure Lengend Snippet: Systemic AAV dissemination, eGFP expression in Schwann cells and inflammation at the site of AAV delivery using SciNDi (A) Dual-IF detection of eGFP and neurofilament light chain (NF-L) in the sciatic nerve trunk following bilateral IM application of PBS (vehicle). Representative images showing eGFP alone (green), NF-L (red), the merge images of eGFP and NF-L, and nuclear marker DAPI (blue). IF procedures and image acquisition were performed as described under E and 2F (nerve trunk and 10× magnified views). (B) Immunohistochemical (IHC) detection of eGFP in the right lumbar L4/L5 spinal ventral horn (VH-R) or both dorsal horns (DH-R and DH-L) after AAV delivery using SciNDi. IHC procedures and image acquisition were performed as described under G–2I. (C) qRT-PCR determination of vector genome copy numbers (AAV2-ITR/2N∗β- tubulin) comparing AAV delivery via IM route or SciNDi (Mean ± s.e.m.; SC: lumbar spinal cord, n = 6; B: whole brain, n = 4–6; and L: liver, n = 6; One- way ANOVA; ns, not significant). (D) qRT-PCR determination of eGFP (transgene) expression (ΔΔCT, relative to PBS injected cohorts). Statistics in A-B (Mean ± s.e.m.; SC: lumbar spinal cord, n = 6; B: whole brain, n = 4–6; and L: liver, n = 6; Mann-Whitney test: ∗= p ≤ 0.05, ns = not significant). (E and F ) Dual-IF co-detection of eGFP in sciatic nerve along with either the Schwann cell marker (S100, in E), or monocyte/macrophage marker (CD68, in F) following bilateral IM application of PBS, and AAV delivery using IM injection or SciNDi. Representative images (20× views) showing eGFP alone (green), S100 alone (red) (in E) or CD68 alone (red) (in F), and the respective merge images along with the nuclear marker DAPI (blue). Antibodies: eGFP (abcam #13970ab, Polyclonal chicken, 1:200), S100 (DAKO #Z0311, rabbit polyclonal, 1:200), CD68 (Novus Biologicals #NBP2-33337, rat mAb FA-11, 1:200). High resolution IF images were acquired using a Zeiss observer inverted microscope equipped with colibri 7 LED illumination. (G–I) Dual-IF co-detection of eGFP and and monocyte/macrophage marker (CD68) in hindlimb femoris muscle following PBS application using SciNDi (in G), and AAV delivery using IM injection (in H) or SciNDi (in I). Representative images (20× view, merge with DAPI, IF procedures and image acquisition were as described under Figure 3 F).

Article Snippet: Antibodies: eGFP (abcam #13970ab, Polyclonal chicken, 1:200), S100 (DAKO #Z0311, rabbit polyclonal, 1:200), CD68 (Novus Biologicals #NBP2-33337, rat mAb FA-11, 1:200).

Techniques: Expressing, Marker, Immunohistochemical staining, Quantitative RT-PCR, Plasmid Preparation, Injection, MANN-WHITNEY, Inverted Microscopy

Journal: STAR Protocols

Article Title: Recombinant adeno-associated virus mediated gene delivery in the extracranial nervous system of adult mice by direct nerve immersion

doi: 10.1016/j.xpro.2022.101181

Figure Lengend Snippet:

Article Snippet: Antibodies: eGFP (abcam #13970ab, Polyclonal chicken, 1:200), S100 (DAKO #Z0311, rabbit polyclonal, 1:200), CD68 (Novus Biologicals #NBP2-33337, rat mAb FA-11, 1:200).

Techniques: Marker, Virus, Recombinant, Plasmid Preparation, Electron Microscopy, Ointment, Sterility, Saline, Transferring, Adhesive, Software